Part:BBa_K1796012
nifX promoted from Paenibacillus sp. WLY78
Function: encode carrier proteins involved in the delivery of FeMoco to the MoFe protein , participate in FeMoco assembly in a chaperone-like function.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 325
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 325
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 325
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 325
- 1000COMPATIBLE WITH RFC[1000]
Parameter of Protein
Number of amino acids: 128
Molecular weight: 13989.1
Theoretical pI: 6.47
Amino acid composition:聽
Ala (A) 15 11.7%
Arg (R) 7 5.5%
Asn (N) 4 3.1%
Asp (D) 3 2.3%
Cys (C) 1 0.8%
Gln (Q) 7 5.5%
Glu (E) 11 8.6%
Gly (G) 10 7.8%
His (H) 4 3.1%
Ile (I) 11 8.6%
Leu (L) 10 7.8%
Lys (K) 6 4.7%
Met (M) 4 3.1%
Phe (F) 7 5.5%
Pro (P) 4 3.1%
Ser (S) 8 6.2%
Thr (T) 6 4.7%
Trp (W) 1 0.8%
Tyr (Y) 0 0.0%
Val (V) 9 7.0%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 13
Atomic composition:Carbon C 621
Hydrogen H 999
Nitrogen N 175
Oxygen O 182
Sulfur S 5
Formula: C621H999N175O182S5Total number of atoms: 1982
Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 0.393, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 0.393, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is K (Lys).
The estimated half-life is: 1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Instability index:The instability index (II) is computed to be 49.46
This classifies the protein as unstable.
Aliphatic index: 96.09
Grand average of hydropathicity (GRAVY): 0.038
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